Refractive index SPR sensor with high sensitivity and wide detection range using tapered silica fiber and photopolymer coating.

This paper introduces a surface plasmon resonance (SPR) sensor using tapered silica fiber and photopolymer coating for enhanced refractive index (RI) detection. Tapering the silica fiber to a diameter of 10 µm ensures the evanescent wave leaks into a 1.8-µm thick photopolymer film, which increases the average waveguide RI and broadens the RI detection range accordingly. A 50-nm thick single-side gold film is coated on the photopolymer film, exciting SPR and causing less light transmission loss than a double-side gold film. The method avoids the complex microfabrication processes of conventional polymer optical fiber SPR sensors, while the waveguide RI can be controlled by altering the curing time of the photopolymer during fabrication. The sensor has an overall sensitivity of 3686.25 nm/RIU, enabling RI detection of 1.333 - 1.493. Moreover, the sensor has an ultrahigh sensitivity of 6422.9 nm/RIU in the RI range of 1.423 - 1.493. The temperature response is about 1.43 nm/°C at 20 - 50 °C, which has little impact on RI detection. Finally, we demonstrate that the sensor can grade the severity of hepatic steatosis by measuring the RIs of cytoplasm/triglyceride emulsions with superior sensing performance.

Lyophilized mRNA-lipid nanoparticle vaccines with long-term stability and high antigenicity against SARS-CoV-2.

Advanced mRNA vaccines play vital roles against SARS-CoV-2. However, most current mRNA delivery platforms need to be stored at -20 °C or -70 °C due to their poor stability, which severely restricts their availability. Herein, we develop a lyophilization technique to prepare SARS-CoV-2 mRNA-lipid nanoparticle vaccines with long-term thermostability. The physiochemical properties and bioactivities of lyophilized vaccines showed no change at 25 °C over 6 months, and the lyophilized SARS-CoV-2 mRNA vaccines could elicit potent humoral and cellular immunity whether in mice, rabbits, or rhesus macaques. Furthermore, in the human trial, administration of lyophilized Omicron mRNA vaccine as a booster shot also engendered strong immunity without severe adverse events, where the titers of neutralizing antibodies against Omicron BA.1/BA.2/BA.4 were increased by at least 253-fold after a booster shot following two doses of the commercial inactivated vaccine, CoronaVac. This lyophilization platform overcomes the instability of mRNA vaccines without affecting their bioactivity and significantly improves their accessibility, particularly in remote regions.

Supercontraction of spider dragline silk for humidity sensing.

The spider dragline silk (SDS) has a supercontraction characteristic, which may cause the axial length of the SDS to shrink up to 50% when the SDS is wet or the relative humidity is higher than 58% RH. In this manuscript, we employ the supercontraction characteristic of the SDS to measure relative humidity. We connect two sections of a single-mode fiber (SMF) and a section of multimode fiber (MMF) with a sandwich structure to fabricate a single-mode-multimode-single-mode (SMS) interferometer. Then we fix the SDS on two SMFs to configure a bow-shaped sensing unit. The increase of environmental humidity will cause the supercontraction of the SDS, which will cause the change of the SDS length. The excellent mechanical properties of the SDS will generate a strong pulling force and change the bending of the arch, whose interference spectrum will shift correspondingly. In this way, we may perform relative humidity sensing. In the relative humidity range of 58% RH to 100% RH, the average sensitivity is as high as 6.213 nm/% RH, higher than most fiber-based humidity sensors. Compared with the traditional sensing structure with humidity-sensitive materials, the proposed sensor improves the sensitivity with environmental friendliness. The results suggest that the SDS can be used for high-sensitivity humidity sensors, and its degradability and biocompatibility also have a vast development space in biochemical sensors.